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Selenium Speciation in Enriched Laminaria Japonica by HPLC-ICP-MS

ZHONG Na1,2, WANG Xiao-Ru3*, YANG Huang-Hao3, CAO Xuan1, WANG Geng4   

    1. College of Chemistry and Chemical Engineering, Ocean University of Qingdao, Qingdao 266003, China;
    2. Qingdao Institute for Drug Control, Qingdao 266071, China;
    3. Key Labortory of Modern Analytical Technology ＆ Standardization of Chinese Medicine of Qingdao, First Institute of Oceanography, State Oceanic Adiministration, Qingdao 266061, China;
    4. College of Chemistry, Shandong University, Jinan 250100, China
  • Received:2007-04-20 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
  • Contact: WANG Xiao-Ru

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Abstract: In the present study, the concentration and speciation of selenium in Laminaria, enriched with selenite during its growth, was performed by establishing a RP-HPLC-ICP-MS method to separate selenite, MeSeCys and SeMet successively. There were three of systems ion-pairing agent and buffer chromatograph: (1) Alltima C8 column, mobile phase: 5 mmol/L citric, 5 mmol/L hexanesulfonic acid(pH 3.5)-methonol(95∶5, volume ratio); (2) Alltima C8 column, mobile phase: water-methnol(98∶2, volume ratio)-0.1%TFA; (3) Alltima C18 column, mobile phase: 20 mmol/L ammonium acetic-methonol(95∶5, volume ratio).Three solvent of 0.1 mol/L HCl, 20 mmol/L Tris-HCl and 20 mmol/L ammonium acetic buffer were used for the extraction of seleno-compound from enriched Laminaria, the major selenospecies determined were selenite, MeSeCys, SeMet, compared with the relative retention time of Se standards.

Key words: Enriched Laminaria, RP-HPLC-ICP-MS, SEC-HPLC-ICP-MS, Total concentration, Speciation, Na2SeO3, MeSeCys, SeMet

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