Studies of Xanthine Oxidation by Xanthine Oxidase Using Microcalorimetry

Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (4): 586.

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Studies of Xanthine Oxidation by Xanthine Oxidase Using Microcalorimetry

LIANG Yi¹, WANG Cun-Xin¹, ZOU Guo-Lin², QU Song-Sheng¹, WU Yuan-Xin³, LI Ding-Huo³

1. Department of Chemistry, College of Life Science, Wuhan University, Wuhan, 430072;
2. College of Life Science, Wuhan University, Wuhan, 430072;
3. Department of Chemical Engineering, Wuhan Institute of Chemical Technology, Wuhan, 430074

Received:1996-03-14 Online:1997-05-24 Published:1997-05-24

Abstract

Abstract: Thermokinetic model of two-parameter initial rate method for faster enzyme-cat-alyzed reactions has been suggested based on the two-parameter theoretical model of conduc-tion calorimeter.Thermokinetics of xanthine oxidation catalyzed by xanthine oxidase wasstudied using microcalorimetry.This faster enzyme-catalyzed reaction obeyed the Michaelis-Menten kinetics, and at 298.15 Kand pH 7.5, the Michaelis constant(K_m) for xanthine was1.04×10^-3 mol·L^-1, which is in agreement with that given in the literature.

Key words: Microcalorimetry, Xanthine oxidase, Faster enzyme-catalyzed reaction, Two-parameter initial rate method, Thermokinetics

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LIANG Yi, WANG Cun-Xin, ZOU Guo-Lin, QU Song-Sheng, WU Yuan-Xin, LI Ding-Huo. Studies of Xanthine Oxidation by Xanthine Oxidase Using Microcalorimetry[J]. Chem. J. Chinese Universities, 1997, 18(4): 586.

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Studies of Xanthine Oxidation by Xanthine Oxidase Using Microcalorimetry

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