Studies on the Inhibiting Action of PCMB to Arginase Using Microcalorimetry

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (5): 744.

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Studies on the Inhibiting Action of PCMB to Arginase Using Microcalorimetry

LIANG Yi¹, WANG Cun-Xin¹, WU Ding-Quan¹, QU Song-Sheng¹, ZOU Guo-Lin²

1. Department of Chemistry, Wuhan University, Wuhan, 430072;
2. College of Life Science, Wuhan University, Wuhan, 430072

Received:1994-05-27 Revised:1994-12-03 Online:1995-05-24 Published:1995-05-24

Abstract

Abstract: Microcalorimetry was used to study the inhibiting action of p-chloromer curibenzoic acid(PCMB)to L-arginine hydrolysis with arginase, PCMB was determined as a competitive irreversible inhibitor and the second-order rate constant of the reaction between PCMB and arginase was k₊₀=92. 17 L/(mol· s)at 298.15 Kand pH 9.4.The properties of the active site of arginase were investigated by using PCMB as a modifying agent.The results of chemical modification reveal that arginase contains 3 reactive cysteinyl residues at most but these residues do not belong to the active site of arginase. The modification of 3 cysteinyl residues by PCMBled to～50% inhibition of arginase activity.

Key words: Microcalorimetry, Arginase, p-Chloromercuribenzoic acid, Irreversible inhibi Tion

CLC Number:

Q556.4

TrendMD:

LIANG Yi, WANG Cun-Xin, WU Ding-Quan, QU Song-Sheng, ZOU Guo-Lin. Studies on the Inhibiting Action of PCMB to Arginase Using Microcalorimetry[J]. Chem. J. Chinese Universities, 1995, 16(5): 744.

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Studies on the Inhibiting Action of PCMB to Arginase Using Microcalorimetry

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