Abstract: A generalized method for surface modification of poly(methyl methacrylate)(PMMA) and polyester materials has been reported to prepare broad-spectrum and subsequently reactive coatings, which have anti-protein and anti-bacterial properties, while avoiding the restriction of introducing specific reaction groups. Taking plexiglass(PMMA) as an example, amphoteric monomer(Sulfobetaine methacrylate, SBMA) was polymerized on PMMA by one-pot and two-step methods, and the one-pot method was optimized for in-depth study by elemental analysis. At the same time, the optimized modification method was verified to be broad-spectrum on the surface of other polyester materials including PBT, PC, PCT and PET. Ellman's assay quantified the density of residual vinyl sulfone groups in the coating to determine the subsequent reactivity of the coating. The anti-biofouling experimental study of the modified PMMA showed that: (1) the anti-BSA adsorption ability of the modified PMMA was evaluated by quartz electronic microbalance(QCM), and the one-pot coating significantly reduced the non-specific adsorption of the protein by 72.3%； (2) the anti-bacterial adhesion ability of the static coating was investigated, and the bacterial adhesion area of the coating was reduced by more than 80% in the case of Bacillus cereus, Escherichia coli and Staphylococcus aureus under the static condition； (3) using the designed flow cell and flow field calculations, the bacterial adhesion resistance was achieved under two flow conditions of shear forces(0.16 dynes/cm2 and 1.6 dynes/cm2).

Key words: One-pot method, Zwitterion; Anti-bacterial adhesion, Flow condition, Shear force