Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics

doi:10.7503/cjcu20200154

Chem. J. Chinese Universities ›› 2020, Vol. 41 ›› Issue (9): 2011.doi: 10.7503/cjcu20200154

• Analytical Chemistry • Previous Articles Next Articles

Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics

DENG Jiewei¹, YANG Yunyun², LIN Li³, LUAN Tiangang¹^,³()

^1.School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China
^2.Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Engineering and Technology Research Center for Ambient Mass Spectrometry, Guangdong Institute of Analysis(China National Analytical Center Guangzhou), Guangdong Academy of Sciences, Guangzhou 510070, China
^3.State Key Laboratory of Biocontrol, South China Sea Bio?Resource Exploitation and Utilization Collaborative Innovation Center, School of Life Sciences, Sun Yat?Sen University, Guangzhou 510275, China

Received:2020-03-19 Online:2020-09-10 Published:2020-09-02
Contact: LUAN Tiangang E-mail:cesltg@mail.sysu.edu.cn
Supported by:
Supported by the National Key Research and Development Program of China(批准号(2018YFD0900604);National Natural Science Foundation of China(批准号(21707171);Guangzhou Municipal Science and Technology Project, China(201804010298)

Abstract

Abstract:

A surface-coated probe nanoelectrospray mass spectrometry(SCP-nanoESI-MS) based lipidomics method was developed for the rapid species identification of Daphnia genus. A microscale surface-coated probe was used to extract and enrich the lipids from Daphnia, and the lipids enriched on the probe were then analyzed by nanoelectrospray ionization mass spectrometry under ambient and open-air conditions. Structural identification of the lipid species was achieved via high resolution MS analysis. By using the proposed method, the lipidomics fingerprints of D. magna and D. pulex were obtained, and the characteristics of lipid compositions from D. magna and D. pulex were also investigated. Rapid classification of D. magna and D. pulex as well as the identification of lipid markers were achieved via chemometrics. The results showed that the proposed SCP-nanoESI-MS lipidomics method has the advantages of fast analysis speed and high sensitivity, and can be used for rapid identification of Daphnia species.

Key words: Solid-phase microextraction, Nanoelectrospray ionization mass spectrometry, Microscale, Lipidomics, Species identification

CLC Number:

O657.6

TrendMD:

DENG Jiewei, YANG Yunyun, LIN Li, LUAN Tiangang. Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics[J]. Chem. J. Chinese Universities, 2020, 41(9): 2011.

Figures/Tables 6

References 21

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No.	Lipid	Molecular formula	Ion	m/z
1	PC (16∶0_16∶1)	C₄₀H₇₈NO₈P	[M+Na]⁺	754.5348
2	PC (16∶0_18∶3)	C₄₂H₇₈NO₈P	[M+Na]⁺	778.5344
3	PC (16∶0_18∶1)	C₄₂H₈₂NO₈P	[M+Na]⁺	782.5657
4	PC (18∶2_18∶2)	C₄₄H₈₀NO₈P	[M+Na]⁺	804.5511
5	TAG (16∶0_16∶4_18∶3)	C₅₃H₈₈O₆	[M+Na]⁺	843.6456
5	TAG (16∶0_16∶4_18∶3)	C₅₃H₈₈O₆	[M+K]⁺	859.6194
6	TAG (16∶0_16∶1_18∶3)	C₅₃H₉₄O₆	[M+Na]⁺	849.6935
6	TAG (16∶0_16∶1_18∶3)	C₅₃H₉₄O₆	[M+K]⁺	865.6695
7	TAG (16∶0_16∶1_20∶5)	C₅₅H₉₄O₆	[M+Na]⁺	873.6917
7	TAG (16∶0_16∶1_20∶5)	C₅₅H₉₄O₆	[M+K]⁺	889.6670
8	TAG (16∶0_16∶1_20∶4)	C₅₅H₉₆O₆	[M+Na]⁺	875.7068
8	TAG (16∶0_16∶1_20∶4)	C₅₅H₉₆O₆	[M+K]⁺	891.6826
9	TAG (18∶1_18∶3_18∶3)	C₅₇H₉₆O₆	[M+Na]⁺	899.7086
9	TAG (18∶1_18∶3_18∶3)	C₅₇H₉₆O₆	[M+K]⁺	915.6817

No.	Lipid	Molecular formula	Ion	m/z
1	PC (16∶0_16∶1)	C₄₀H₇₈NO₈P	[M+Na]⁺	754.5348
2	PC (16∶0_18∶3)	C₄₂H₇₈NO₈P	[M+Na]⁺	778.5344
3	PC (16∶0_18∶1)	C₄₂H₈₂NO₈P	[M+Na]⁺	782.5657
4	PC (18∶2_18∶2)	C₄₄H₈₀NO₈P	[M+Na]⁺	804.5511
5	TAG (16∶0_16∶4_18∶3)	C₅₃H₈₈O₆	[M+Na]⁺	843.6456
5	TAG (16∶0_16∶4_18∶3)	C₅₃H₈₈O₆	[M+K]⁺	859.6194
6	TAG (16∶0_16∶1_18∶3)	C₅₃H₉₄O₆	[M+Na]⁺	849.6935
6	TAG (16∶0_16∶1_18∶3)	C₅₃H₉₄O₆	[M+K]⁺	865.6695
7	TAG (16∶0_16∶1_20∶5)	C₅₅H₉₄O₆	[M+Na]⁺	873.6917
7	TAG (16∶0_16∶1_20∶5)	C₅₅H₉₄O₆	[M+K]⁺	889.6670
8	TAG (16∶0_16∶1_20∶4)	C₅₅H₉₆O₆	[M+Na]⁺	875.7068
8	TAG (16∶0_16∶1_20∶4)	C₅₅H₉₆O₆	[M+K]⁺	891.6826
9	TAG (18∶1_18∶3_18∶3)	C₅₇H₉₆O₆	[M+Na]⁺	899.7086
9	TAG (18∶1_18∶3_18∶3)	C₅₇H₉₆O₆	[M+K]⁺	915.6817

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Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics

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