Special Surface Enhanced Raman Scattering on Lung Cancer Tissues Based on Au/Cu Nanorods Substrate†

doi:10.7503/cjcu20170233

Abstract

Abstract:

Au/Cu nanorods were prepared by mixing method. The influence of doping copper ions on the plasmon resonance absorption and morphology of gold nanorods was investigated. The plasmon resonance Raman enhancement effect on Au/Cu alloy nanorods was detected. The Raman molecular vibration information of lung cancer tissues and corresponding non-tumor normal tissues were compared on Au/Cu alloy nanorods substrate. Surface enhanced Raman scattering(SERS) analysis of lung cancer tissues and corresponding non-tumor normal tissues demonstrate that the Raman peaks of cancer tissues are stronger compared to non-tumor normal tissues because the SERS signals of protein molecules in lung adenocarcinoma are influenced by their spatial conformation and molecular symmetry. The results suggest that tissue carcinogenesis increases the symmetry of the molecular structure and the Raman peaks near 1250, 1344, 1408, 1568, 1608 and 2560 cm^-1 are relevant to amino-compounds of amide Ⅱ in protein, C—H bending vibration, symmetrical variable angle vibration of CH₃ in nucleic acids, inert ring vibration of tryptophan in protein and C—O stretching vibration of amide Ⅰ band in protein with intermolecular antiparallel β-fold and sulfhydryl(S—H) stretching vibration of proteins, respectively. The Raman peak near 2936 cm^-1 is generated by the joint action of the symmetric stretching vibration of CH₂ and the antisymmetric stretching vibration of CH₃ in protein. Herein, SERS based on Au/Cu alloy nanorods as substrate is promising to be an effective method for the detection of lung cancer.

Key words: Au nanorods, Copper ion doping, Surface enhanced Raman spectrum, Lung cancer tissue

CLC Number:

O657.37
O614

TrendMD:

TONG Ti*, ZHAO Yangyang, FU Yilin, SHAN Guiye. Special Surface Enhanced Raman Scattering on Lung Cancer Tissues Based on Au/Cu Nanorods Substrate^†[J]. Chem. J. Chinese Universities, 2017, 38(9): 1536.

Figures/Tables 5

Fig.1 SEM image(A) and XRD pattern(B) of Au/Cu NRs

Fig.2 Absorption spectra of Au NRs(a), Au/Cu NRs at Cu2+ molar ratio of 0.1%(b) and 1.0%(c)(A) and TEM images of Au NRs(B) and Au/Cu NRs(C)

Fig.3 SERS spectra of 4-ATP molecules adsorbed on Au NRs(a) and Au/Cu NRs(b)

Fig.4 Photos of the surface of Au/Cu NRs(A) and Au/Cu NRs coated by lung tumor tissues(B)

Fig.5 SERS spectra of different lung tissues on different substrates(A) a. Lung cancer tissue on glass substrate; b. normal lung tissue on Au/Cu NRs(0.1% of Cu2+) substrate; c. normal lung tissue on the Au/Cu NRs(1.0% of Cu2+) substrate; d. lung cancer tissue on Au/Cu NRs(0.1% of Cu2+) substrate; e. lung cancer tissue on Au/Cu NRs(1.0% of Cu2+) substrate. (B) a. Normal tissue on Cu@Au NRs substrate; b. cancer tissue on Au/Cu NRs substrate(0.1% of Cu2+); c. cancer tissue on Au/Cu NRs substrate(1.0% of Cu2+).

References 32

[1]	Siegel R. L., Miller K. D., Jemal A., CA. Cancer J. Clin., 2016, 66(1), 7—30
[2]	Neri S., Miyashita T., Hashimoto H., Suda Y., Ishibashi M., Kii H., Watanabe H., Kuwata T., Tsuboi M., Goto H., Menju T., Sonobe M., Date H., Ochiai A., Ishii G., Cancer Lett., 2017, 395, 20—30
[3]	Esfahlan R. J., Seidi K., Monfaredan A., Irannejad V. S., Abbasi M. M., Karimian A., Yousefi B., Gene,2017, 613, 14—19
[4]	Ribaut C., Loyez M., Larrieu J. C., Chevineau S., Lambert P., Remmelink M., Wattiez R., Caucheteur C., Biosens. Bioelectron., 2017, 92, 449—456
[5]	Wang Z. L., Qiao R. R., Tang N., Lu Z. W., Wang H., Zhang Z. X., Xue X. D., Huang Z. Y., Zhang S. R., Zhang G. X., Li Y. P., Biomaterials,2017, 127, 25—35
[6]	Larsen J. E., Cascone T., Gerber D. E., Heymach J. V., Minna J. D., Cancer J., 2011, 17(6), 512—527
[7]	Liu S. Y., Mok T., Wu Y. L., Cancer,2015, 121, 3089—3096
[8]	Tan Y. H., Xiao X., Yao J. N., Han F., Lou H. Y., Luo H., Liang G. Y., Yaacov B. D., Pan W. D., Chem. Res. Chinese Universities, 2017, 33, 80—86
[9]	Deng L., Wang Y. X., Liu C., Hu D. J. J., Shum P. P., Su L., Opt. Commun., 2016, 372, 113—117
[10]	Luo Z. X., Loo B. H., Peng A. D., Ma Y., Fu H. B., Yao J. N., J. Raman Spectrosc., 2011, 42(3), 319—323
[11]	Aroca R. F., Alvarez-Puebla R. A., Pieczonka N., Sanchez-Cortez S., Garcia-Ramos J. V., Adv. Colloid Interface Sci., 2005, 116, 45—61
[12]	Pettinger B., Schambach P., Scott N. R., Mol. Phy., 2010, 108(16), 2039—2059
[13]	Chen D., Copeland C., Majumdar D., Roszak S., Leszczynski J., Struct. Chem., 2017, 28(2), 379—388
[14]	Zhang D. M., Ansar S. M., Vangala K., Jiang D. P., J. Raman Spectrosc., 2010, 41(9), 952—957
[15]	Park W. H., Kim Z. H., Nano Lett., 2010, 10(10), 4040—4048
[16]	Tsekenisa G., Filippidoub M. K., Chatzipetrouc M., Tsoutib V., Zergiotic I., Chatzandroulis S., Sens. Actuators B, 2015, 208, 628—635
[17]	Schneider E., Clark D. S., Biosens. Bioelectron., 2013, 39, 1—13
[18]	Turnlund J. R., Am. J. Clin. Nutr., 1998, 67, 960—964
[19]	Won Y. H., Huh K., Stanciu L. A., Biosens. Bioelectron., 2011, 26(11), 4514—4519
[20]	Liu J. S., Kan C. X., Li Y. L., Xu H. Y., Ni Y., Shi D. N., Plasmonics,2015, 10(1), 117—124
[21]	Zhang L. Y., Chi Y. N., Shan G. Y., Chen Y. W., Li N., Chem. J. Chinese Universities, 2016, 37(7), 1239—1244
	(张龄月, 迟亚楠, 单桂晔, 陈艳伟, 李娜.高等学校化学学报,2016, 37(7), 1239—1244)
[22]	Shan G. Y., Zheng S. J., Chen S. P., Chen Y. W., Liu Y. C., Colloids Surf. B, 2013, 102, 327—330
[23]	Yuan C., Zhang K., Zhang Z., Wang S., Anal. Chem., 2012, 84, 9792—9801
[24]	Wang Y., Deprince A. E., Gray S. K., Lin X. M., Pelton M., J. Phys. Chem. Lett., 2012, 1, 2692—2698
[25]	Nie Z., Fava D., Kumacheva E., Zou S., Walker G. C., Rubinstein M., Nat. Mater., 2007, 6, 609—614
[26]	Thomas K. G., Barazzouk S., Ipe B. I., Joseph S. T. S., Kamat P. V., J. Phys. Chem. B, 2004, 108, 13066—13068
[27]	Varghese N., Vivekchand S. R. C., Govindaraj A., Rao C. N. R., Chem. Phys. Lett., 2008, 450, 340—344
[28]	Moussawi R. N., Patra D., J. Phys. Chem. C, 2015, 119, 19458—19468
[29]	Lu L. L , Xia Y. S., Anal. Chem., 2015, 87, 8584—8591
[30]	Wang J., Dong B., Chen B., Xu S., Zhang S., Yu W., Xu C. X., Song H. W., Dalton Trans., 2013, 42, 11548—11558
[31]	Kim K., Lee H. B., Choi J. Y., J. Phys. Chem. C, 2011, 115(43), 21047—21055
[32]	Feng S., Lin J., Huang Z., Chen G., Chen W., Wang Y., Zeng H., Appl. Phys. Lett., 2013, 102, 043702