Chem. J. Chinese Universities ›› 2016, Vol. 37 ›› Issue (10): 1915.doi: 10.7503/cjcu20160342

• Polymer Chemistry • Previous Articles     Next Articles

Investigation on Synthesis of Bletilla Striata Polysaccharides Amphiphilic Polymer and Drug-loaded Delivery System

GUAN Qingxiang, ZHANG Guangyuan, SUN Dandan, SUN Shilin, SUN Cheng, LIU Xin*(), HAN Bing*()   

  1. School of Pharmacy, Jilin University, Changchun 130021, China
  • Received:2016-05-16 Online:2016-10-10 Published:2016-08-31
  • Contact: LIU Xin,HAN Bing E-mail:liux@jlu.edu.cn;hanb@jlu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.81401513) and the Graduate Innovation Fundation of Jilin University, China(No.2016225)

Abstract:

Stearic acid(SA)-modified Bletilla Striata polysaccharides(BSPS) amphiphilic copolymers(SA-BSPS) were prepared. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy(1H NMR) were used to describe SA-BSPS group. The substitued degree of the SA-BSPS group was determined by the peak area of lH NMR. Docetaxel-loaded SA-conjugated BSPS(DTX-SA-BSPS) copolymer micelles were formulated and characterized in terms of particle diameter, Zeta potential, drug loading capacity(LC) and encapsulation efficiency(EE). Further, the uptake of rhodamine B-labeled SA-BSPS copolymer micelles and antitumor activity in vitro on human liver cancer cell line (HepG2) were carried out. The results indicated that SA was successfully conjugated with BSPS and the substitued degree of the SA-BSPS group was 12.94%. DTX-SA-BSPS copolymer micelles had an average size of (97.01±3.17) nm with Zeta potential in the range of (-19.56±0.22) mV. The drug encapsulation and loading capacity were (81.11±0.18)% and (9.13±0.17)% respectively. The average cell line viability of blank SA-BSPS polymer micelles were (78.82±3.25)% at 0.5 μg/mL after 72 h incubation. Fluorescent intensity of HepG2 cells incubated for 4 h with rhodamine B-labeled SA-BSPS copolymer micelles was higher than that of rhodamine B and fluorescent intensity exhibited an increase trend when incubation time were prolonged.

Key words: Bletilla Striata polysaccharide, Amphiphilic copolymer, Polymer micelle, Drug loading capacity, Encapsulation efficiency

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