CJCU

Chem. J. Chinese Universities

• 研究论文 • Previous Articles     Next Articles

Purification and Preparation of Active Subunits of C-phycocyanin by a Protocol of Liquidphase Isoeletric Focusing

HUANG Zhi1,2, YANG Fang1, ZHENG Wen-Jie1, GUO Bao-Jiang2*   

  1. 1. Department of Biotech, Life Science College, Jinan University, Guangzhou 510632, China;
    2. Biotechnology Research Institute, South China Normal University, Guangzhou 510631, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10
  • Contact: GUO Bao-Jiang

PDF (PC)

Abstract: A process of liquidphase isoeletric focusing(LP-IEF) was developed and conducted in a 8.0 mol/L urea medium in Rotofor system for isolation, purification and preparation the α and β subunits of C-PC(αβ)3 purified from Spirulina platensis. After two steps of pre- and re- fractionating, collected fractions with α or β subunit were identified and characterized by SDSPAGE and slab ultra thin IEF. Its absorption and fluorescence spectra were determined. The results show that the α and β subunits were concentrated at about pH=4.9 and pH=4.1 respectively and the total recovery of α and β subunits was 392%. The α and β subunits of C-PC with a high purity as well as activity maintaining were obtained easily by this LP-IEF protocol, suggesting that it should be an ordinary but effective method for purification and preparation of protein subunits with different pI values.

Key words: Protein subunit, Liquidphase preparative IEF(LP-IEF), Isoelectric point(pI), C-phycocyanin(C-PC), Preparative electrophoresis

CLC Number: 

TrendMD: