Abstract: The influence of Fmoc-protected amino acid, substitution and particle size of Wang resin and stirring modes on the condensation reaction efficiency of Fmoc-protected amino acid and Wang resin were studied. It is found that the molecular size of Fmoc-protected amino acid affected the efficiency of condensation reaction, the small the amino acid is, the higher the efficiency of condensation reaction will be; when the substitution of Wang resin is higher, Fmoc-protected amino acid conjoined before hindering the later condensation reaction, so that the hindered condensation reaction efficiency decrease.

Key words: Solid phase synthesis, Wang resin, Fmoc-protected amino acid, Active ester method