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Separation, Purification and Structure Analysis of an Extracellular Polysaccharides from Antarctic Bacterium Pseudoalteromonas sp. S-15-13

LI Jiang1*, SONG Guo-Qiang2, CHEN Kao-Shan1,3, LI Guang-You1   

    1. Key Laboratory of Marine Bio-active Substances, First Institute of Oceanorgarphy, State Oceanic Administration, Qingdao 266061, China;
    2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
    3. College of Life Sciences, Shandong University, Jinan 250100, China
  • Received:2007-09-28 Revised:1900-01-01 Online:2008-06-10 Published:2008-06-10
  • Contact: LI Jiang

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Abstract: An immunoactive extracelluar polysaccharide was isolated from Pseudoalteromonas sp. S-15-13, a bacterium screened from Antarctic sea-ice. In order to accurately define the structure of the Antarctic bacterial EPSs and to relate these findings to the function of these molecules in the natural environment, purification and structural analysis of S-15-13 EPSs was carried out. Exopolysaccharide fractions were extracted and were purified by DEAE-Sephadex A-50 ion-exchange and Sephadex G-100 gel chromatography to give EPS-Ⅱ. The EPS-Ⅱwas eluted as a single peak in HPLC analysis, indicating that the homogeneity and purity of EPS-Ⅱwere suited to the structural analysis. The molecular weight of the EPS-Ⅱ was determined as 62000 by the HPLC method. Sugar composition analysis, methylation analysis and one-dimensional and two-dimensional NMR spectroscopies reveal that the main structure of EPS-Ⅱwas a 1,2 linking mannan with fewer 1,2,6-linking branch, a new extracelluar polysaccharide from Antarctic bacterium.

Key words: Extracelluar polysaccharide, Antarctic bacterium Pseudoalteromonas sp. S-15-13, Separation and purification

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