Abstract: Acting on a broad spectrum of extracellular, intracellular, and membrane-associated substrates, the matrix metalloproteinases(MMPs) are critical to the biological processes of organisms. And when aberrantly expressed, many pathological conditions may be born or exacerbated. Several natural flavonoids were used as the inhibitors of MMP-16, and the fluorometric titration and UV-Vis absorption spectra were performed to reveal the recognition and inhibition mechanism between them, large differences at binding model, stoichiometry and antioxidation were clarified between them.

Key words: Matrix metalloproteinases(MMPs), Natural medicine inhibitor, Molecular recognition and mechanism, Flavonoids