Studies on the Binding Geometry of Intercalation of 4-(2-Diethylamino-ethylamino)-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile to DNA by Molecular Spectra

Chem. J. Chinese Universities

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Studies on the Binding Geometry of Intercalation of 4-(2-Diethylamino-ethylamino)-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile to DNA by Molecular Spectra

YANG Yuan-Yuan¹, ZHANG Zhi-Chao¹, SHENG Hui², LIU Feng-Yu¹, QIAN Xu-Hong^1,3, XU Qin¹, ZHANG Jing^1,4

1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China;
2. The First Hospital of Jilin University, Changchun 130021, China;
3. Shanghai Key Laboratory of Chemical Biology, East China University of Science and Technology, Shanghai 200237, China;
4. State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071, China

Received:2006-05-11 Revised:1900-01-01 Online:2007-03-10 Published:2007-03-10
Contact: ZHANG Zhi-Chao

Abstract

Abstract: The binding geometry of a heterocyclic compound, 4-(2-diethylamino-ethylamino)-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile(A1) to CT DNA was studied by using molecular spectroscopy. Deduced from SYBR Green-DNA melt curve, fluorescence spectroscopy, UV-Vis spectroscopy and circular dichroism(CD), there were two different interaction mechanisms involved in the whole interaction process depending on the the molar ratios of A1 to CT DNA base pairs. The binding geometry of A1 to CT DNA was investigated by the induced CD spectroscopy based on the different n(A1)/n(CT DNA) ratio. The value n(A1)/n(CT DNA)=0.20 was the turning point; when n(A1)/n(CT DNA)≤0.20, the intercalation orientation of A1 to the dyad axis of DNA double helix was heterogeneous and stacking of A1 on the surface helix of DNA was available when n(A1)/n(CT DNA)>020.

Key words: Intercalator, Geometry, DNA conformation, Circular dichroism

CLC Number:

O657
Q523.4

TrendMD:

YANG Yuan-Yuan¹, ZHANG Zhi-Chao¹, SHENG Hui², LIU Feng-Yu¹, QIAN Xu-Hong^1,3, XU Qin¹, ZHANG Jing^1,4. Studies on the Binding Geometry of Intercalation of 4-(2-Diethylamino-ethylamino)-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile to DNA by Molecular Spectra [J]. Chem. J. Chinese Universities, doi: .

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Studies on the Binding Geometry of Intercalation of 4-(2-Diethylamino-ethylamino)-8-oxo-8H-acenaphtho[1,2-b]pyrrole-9-carbonitrile to DNA by Molecular Spectra

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