Construction and Evaluation of Continuous Two\|dimensional Open\|tubular Ion Exchange/Reversed Phase Monolithic Column Capillary Electrochromatography System

Chem. J. Chinese Universities ›› 2011, Vol. 32 ›› Issue (1): 44.

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Construction and Evaluation of Continuous Two\|dimensional Open\|tubular Ion Exchange/Reversed Phase Monolithic Column Capillary Electrochromatography System

ZHU Ya-Xian, ZHANG Ling-Yi*, LEI Wen, ZHANG Wei-Bing*

Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, China

Received:2010-05-25 Revised:2010-09-26 Online:2011-01-10 Published:2010-12-11
Contact: ZHANG Wei-Bing E-mail:weibingzhang@dicp.ac.cn
Supported by:
国家“九七三”计划项目(批准号: 2007CB914100)和国家自然科学基金(批准号: 21075039)资助.

Abstract

Abstract: A continuous two-dimensional open-tubular ion exchange / reversed phase monolithic column capillary electrochromatography system was developed. Reversed phase monolithic column was firstly synthesized in the end part of the capillary, then N-(3-(trimethoxysilyl)-propyl)-ethlenediamine was surface coated to the other part of the capillary. The separation of seven organic acids verified the effect on the separation of ion exchange in the open-tubular capillary. Rhizoma gastrodiae was separated to evaluate the system, and 34 peaks were recognized. In the new electrochromatography system, electroosmotic flow, electrophoresis flow and interaction between solute and stationary phase, which all devote to adjusting the selectivity of the separation. This system is designed for complex samples with highly hydrophobicity components and highly hydrophilicity components simultaneous. This new column system combining advantages of multidimensional separation and adjusted stationary phase selectivity is suitable for the analysis method development of complicated samples.

Key words: capillary electrochromatography, two-dimensional separation, monolithic column

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ZHU Ya-Xian, ZHANG Ling-Yi*, LEI Wen, ZHANG Wei-Bing*. Construction and Evaluation of Continuous Two\|dimensional Open\|tubular Ion Exchange/Reversed Phase Monolithic Column Capillary Electrochromatography System[J]. Chem. J. Chinese Universities, 2011, 32(1): 44.

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Construction and Evaluation of Continuous Two\|dimensional Open\|tubular Ion Exchange/Reversed Phase Monolithic Column Capillary Electrochromatography System

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