Chemical Modification of Tryptophan Residues in Human Plasminogen

Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (5): 744.

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Chemical Modification of Tryptophan Residues in Human Plasminogen

ZHAO Chen-Guang¹, LIU Lan-Ying¹, QIU Ai-Dong², LIU Shu-Ying²

1. Department of Molecular Biology, Jilin University, Changchun, 130023;
2. Institution of Biological Production, Hygiene Mnistry, Changchun, 130062

Received:1996-07-11 Online:1997-05-24 Published:1997-05-24

Abstract

Abstract: Tryptophan(Trp) residues in human plasminogen(HPg) was modified by N-bro-mossuccinimide(NBS).The results indicate that there are 19 tryptophan residues in HPg.Five tryptophan residues are located on the surface of the enzymogen, two of them are fast reactive residues.Among all the Trp residues, one is essential for the activity of the enzyme and may be located at the substrate binding site of the enzyme.The spectra of circular dichroism(CD) of HPg are changed after treatment with NBS.

Key words: Human plasminogen(HPg), Tryptophan(Trp), Chemical modification

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ZHAO Chen-Guang, LIU Lan-Ying, QIU Ai-Dong, LIU Shu-Ying. Chemical Modification of Tryptophan Residues in Human Plasminogen[J]. Chem. J. Chinese Universities, 1997, 18(5): 744.

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Chemical Modification of Tryptophan Residues in Human Plasminogen

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