Synthesis of the IS3 Segment of the Rat Brain Sodium Ion Channel

Chem. J. Chinese Universities ›› 1997, Vol. 18 ›› Issue (5): 734.

• Articles • Previous Articles Next Articles

Synthesis of the IS3 Segment of the Rat Brain Sodium Ion Channel

MIAO Zhen-Wei, JIANG Yun, LI Xiang-Qun, XU Xiao-Jie, TANG You-Qi

College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871

Received:1996-05-28 Online:1997-05-24 Published:1997-05-24

Abstract

Abstract: The IS3 segment, a peptide with 22 amino acid residues in length, of the rat brain sodium ion channel was synthesized by the fragment condensation using the phenacylester as the temporary protection group of a-carboxyl group.After removal of the protection group,the crude product was purified by HPLCfirst on Protein Parck column and then on C18 column, using 40% acetonitrile in 0.1 mol/L NH₄HCO₃ aqueous solution as the mobile phase.The purified IS3 segment was confirmed by amino acid analysis and FAB-MS.

Key words: Phenacylester, Peptide synthesis, IS3 segment, Fragment condensation

TrendMD:

MIAO Zhen-Wei, JIANG Yun, LI Xiang-Qun, XU Xiao-Jie, TANG You-Qi. Synthesis of the IS3 Segment of the Rat Brain Sodium Ion Channel[J]. Chem. J. Chinese Universities, 1997, 18(5): 734.

[1]	ZONG Liang, DONG Junjun, CHEN Jing, LI Jian, SUI Shaohui, WEI Yifei, ZHANG Ming. Preparation of Oxime Cesium Salts and Its Function as Cleaving Agents in Peptide Synthesis^† [J]. Chem. J. Chinese Universities, 2016, 37(7): 1328.
[2]	WANG Yehai, KONG Yifu, CHEN Chenchen, LI Yiming. Chemical Synthesis of K33 Acetylated SUMO Protein^† [J]. Chem. J. Chinese Universities, 2016, 37(11): 1987.
[3]	WANG Hua, Zhao-Mi-Feng, Li-Gang, Huang-Yi-Bing, Hou-Rui-Zhen, Ceng-Hong-Bin, Xu-Li, Zhang-Xua-Zhong. Chemical Synthesis and Esterification of a Precursor Dipeptide of RGD as a Site for Cell Adhesion [J]. Chem. J. Chinese Universities, 2006, 27(2): 240.
[4]	FANG Can-Liang, ZHAO Rui, LIU Yang, YU Xiao, XIONG Shao-Xiang, LIU Guo-Quan . Screening of Affinity Peptide Ligands from Combinatorial Peptide Libraries [J]. Chem. J. Chinese Universities, 2003, 24(1): 52.
[5]	WANG Rui, HU Xiao-Yu, MA Ya-Ping, Li Du. Studies on the Solution Conformation of Angiotensin Ⅱ [J]. Chem. J. Chinese Universities, 1998, 19(11): 1758.
[6]	ZHANG Ruo-Heng, WANG He, XU Xiao-Jie, TANG You-Qi. Solid Phase Peptide Synthesis of Designed Yeast Transcriptional Activator Ac [Q²³⁶,Q²⁴²]GCN4(226─252)─GGC─NH₂ and Its Dimerization [J]. Chem. J. Chinese Universities, 1996, 17(5): 747.
[7]	TIAN Gui-Ling, LIU Ying, WANG Hong, Ye Yun-Hua. Enzymatic Synthesis of Peptides Using N-Protected Racemic Amino Acides as Carboxyl Components [J]. Chem. J. Chinese Universities, 1996, 17(1): 55.
[8]	HU Xiao-Yu, WANG Rui, JIA Qing, JI Hong. Design and Synthesis of Deltorphin Ⅰ, Ⅱ Analogues [J]. Chem. J. Chinese Universities, 1995, 16(10): 1569.
[9]	ZHANG Ran, WANG Yu-Hong, ZHU Zhi-Hua, GUO Li-Li, XU Jian-Ping, WANG Zong-Mu, LI Wei. Solid Phase Synthesis and Biological Activities of α-h-ANF and Its Analogs [J]. Chem. J. Chinese Universities, 1992, 13(10): 1247.
[10]	Wang Ensi, Xu Jianping, Wang Zongmu, Li Wei, S. Funakoshi, H. Yajima. Solid Phase Synthesis of the Human Trypsin Inhibitor [J]. Chem. J. Chinese Universities, 1990, 11(8): 837.
[11]	Xue Chubiao, Li Chongxi, Xing Qiyi, Dong Minghui. The Use of TTT Active Amide Method in Solid-phase Peptide Synthesis——The Synthesis of the Growth Hormone Releasing Peptide and Its Biological Activity [J]. Chem. J. Chinese Universities, 1988, 9(5): 461.

Synthesis of the IS3 Segment of the Rat Brain Sodium Ion Channel

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments