水溶性香豆素荧光底物的制备及在液滴数字式检测中的应用

匡小军, 伊京伟, 方晓霞, 赖东梅, 徐宏

Preparation of Water-soluble Coumarin Fluorescent Substrate and Its Application in Droplet Based Digital Detection

KUANG Xiaojun, YI Jingwei, FANG Xiaoxia, LAI Dongmei, XU Hong

Fig.4 Droplet based digital detection of SP?AKP using 4?MUP and C?4 (A) The fluorescence distribution of droplets and fluorescence images(insert pictures) of SP?AKP at λT=0.0007(A1), 0.007(A2), 0.069(A3), and 0.312(A4) using 4?MUP after 10 min incubation. (B) The fluorescence distribution of droplets and fluorescence images (insert pictures) of SP?AKP at λT=0.0007(B1), 0.007(B2), 0.069(B3), and 0.312(B4) using C?4 after 20 min incubation. The experimental λTversus theoretical λT using 4?MUP(C) and C?4(E). The ordinate and horizontal axis indicate the experimental λT and the theoretical λT, respectively. The experimental λTversus SP?AKP concentration using 4?MUP(D) and C?4(F).