水溶性香豆素荧光底物的制备及在液滴数字式检测中的应用

匡小军, 伊京伟, 方晓霞, 赖东梅, 徐宏

Preparation of Water-soluble Coumarin Fluorescent Substrate and Its Application in Droplet Based Digital Detection

KUANG Xiaojun, YI Jingwei, FANG Xiaoxia, LAI Dongmei, XU Hong

Fig.3 Characterization of 4?MUP and C?4 in droplets over time (A) The fluorescence distribution of droplets and fluorescence images(insert pictures) after 0 min(A1), 5 min(A2), 10 min(A3), and 30 min(A4) incubation of 4?MUP substrate under λ=0.04. (B) The fluorescence distribution of droplets and fluorescence images(insert pictures) after 0 min(B1), 10 min(B2), 20 min(B3), and 60 min(B4) incubation of C?4 substrate under λ=0.55. Summary of florescence distribution after different incubation time using 4?MUP(C) and C?4(E). Different color indicates different incubation times after droplet generation. The experimental λ of 4?MUP(D) and C?4(F) over time.