水溶性香豆素荧光底物的制备及在液滴数字式检测中的应用

匡小军, 伊京伟, 方晓霞, 赖东梅, 徐宏

Preparation of Water-soluble Coumarin Fluorescent Substrate and Its Application in Droplet Based Digital Detection

KUANG Xiaojun, YI Jingwei, FANG Xiaoxia, LAI Dongmei, XU Hong

Fig.1 Basic property characterization of 4?MU, C?2, 4?MUP, C?4 and SP?AKP （A） The fluorescence spectra and partition coefficients（lg D） of 4?MU and C?2； （B） the fluorescence intensity standard curves of 4?MU and C?2； （C） the Michaelis?Menten equation of corresponding substrate C?4 and 4?MUP； （D） the catalytic activity comparison of AKP and SP?AKP. The concentrations of AKP and SP?AKP are 1×10-6 mg/mL and 12.17×10-6 mg/mL， respectively.