重组酶聚合酶扩增:扩增原理及性能分析

Recombinase Polymerase Amplification： from Principle to Performance

LI Xianming, ZHENG Ting, GAO Lu, LI Feng, HOU Xiandeng, WU Peng

Table 2 Comparison of current isothermal amplification methods

Assay^a	Primer number	Temperature/℃	Reaction/min	Detection formats^b	Target	Reagent format	Multiplex	Ref.
NASBA	2	41	105	RTF, NALF	RNA(DNA)	Liquid	Y	[56]
TMA	2	60	140	RTF	RNA(DNA)	Liquid	Y	[57]
SMART	2	41	140	RTF	RNA, DNA	Liquid	N/A	[58]
LAMP	4	60—65	60—90	RTF, NALF, RTT, TE	RNA, DNA	Liquid	N/A	[59,60]
CPA	4	65	65	RTF, NALF	DNA	Liquid	N/A	[61]
RCA	1, 2	65	60	RTF	RNA, DNA	Liquid	N/A	[62]
RAM	1	63	120—180	RTF	RNA, DNA	Liquid	N/A	[63]
SDA	2	37	120	RTF, NALF	RNA, DNA	Liquid	Y	[64,65]
NEAR	4	55	10	RTF, NALF	RNA, DNA	Liquid	Y	[66]
NEMA	4	65	30	NALF	DNA	Liquid	N/A	[67]
ICA	2	60	60	RTF	DNA	Liquid	N/A	[68]
EXPAR	1	55	10—20	RTF, NALF	DNA	Liquid	Y	[69,70]
HDA	2	65	75—90	RTF, NALF	RNA, DNA	Liquid	Y	[71,72]
RPA	2	30—42	20	RTF, NALF	RNA, DNA	Liquid, lyophilized	Y	[10,73]