重组酶聚合酶扩增:扩增原理及性能分析

Recombinase Polymerase Amplification： from Principle to Performance
LI Xianming, ZHENG Ting, GAO Lu, LI Feng, HOU Xiandeng, WU Peng
Table 1 Functional substances involved in recombinase polymerase amplification
CategoryStrategy IStrategy IIFunction description
RecombinaseuvsXRecADNA?dependent ATPase, the key protein for DNA strand exchange

ssDNA binding

proteins

gp32SSB

Melting the secondary structure of primers to decrease undesirable

interactions

Accessory proteinsuvsYRecF, RecOAssisting the loading of recombinase on primer
Crowding agentsPEGs, dectran, ficollAccelerating the RP process and primer extending
PolymeraseT4 polymerase, Bsu DNA polymerase I, etc.

Extending the primer and displacement of the complementary strand

of templates

ATP regenerationPhosphocreatine and creatineTransforming ADP into ATP
Reverse transcriptaseMu?MLV reverse transcriptaseConverting RNA templates to ssDNA