Chem. J. Chinese Universities ›› 2012, Vol. 33 ›› Issue (07): 1407.doi: 10.3969/j.issn.0251-0790.2012.07.008

• Analytical Chemistry • Previous Articles     Next Articles

Synthesis and Application of an Efficient and Sensitive DNA Fluorescent Probe

WU Tong, PENG Xiao-Jun, HU Ming-Ming, LIU Fei, FAN Jiang-Li   

  1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China
  • Received:2011-11-21 Online:2012-07-10 Published:2012-07-10

Abstract: A novel thiazole orange dimmer derivative Bi-TO3 was developed. The interaction of Bi-TO3 with DNA was investigated through the absorption, emission, circular dichroism spectroscopy and live-cell imaging experiments. As a specific probe for DNA, Bi-TO3 itself was almost non-fluorescent(quantum yield<0.001%) in buffer. Upon binding to DNA, the fluorescence of Bi-TO3 increased approximately 950-fold, even without interferences of responding to RNA, BSA, viscosity, etc. Absorption and circular dichroism spectra showed that Bi-TO3 associated with DNA in a minor groove binding mode. The emission detection limit was 13.3 ng/mL, which manifested that Bi-TO3 could detect trace DNA in solution. Moreover, Bi-TO3 could be applied for live-cell fluorescence imaging with relatively low concentrations of 6-12 μmol/L.

Key words: Thiazole orange dimmer, Fluorescent probe, DNA quantitative detection, Live-cell fluorescence imaging

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